Dozens of ancient coins, oil lamps, jewelry and Jewish ritual objects were found in a police raid on a home in the village of Beit Ula, northwest of Hebron, on Wednesday.
The stolen antiquities were valued at tens of thousands of dollars, according to officials.
Police detained the homeowner, a Palestinian man in his 50s.
Archaeologists who examined the cache said the antiquities were from a broad cross-section of historical periods, from Hellenistic to Roman and Byzantine times, and on into the Middle Ages.
The detainee will be brought before a military court Thursday to have his remand extended, police said.
In April, a Palestinian man suspected of smuggling hundreds of antiquities was arrested by police in a village outside Nablus in the West Bank.
A joint police and Civil Administration operation found a trove of antiquities mostly dating to the Hellenistic, Second Temple and Roman periods whose value was estimated at thousands of dollars.
The 50-year-old man was detained on suspicion of antiquities trafficking and an investigation was launched to determine whether he was part of a larger network of looters and dealers operating in the West Bank and Israel.
Ilan Ben Zion contributed to this report.