Two brothers and their sister, aged 3, 4, and 8, respectively, were fatally injured Thursday morning when they were hit by a car in the Shuafat refugee camp of East Jerusalem.
United Hatzalah paramedics who arrived at the scene reported eyewitnesses saying the children were hit as they were walking to school.
Red Crescent ambulances collected the children and took them to a checkpoint, where they were handed over to Magen David Adom ambulances.
The girl was taken to the Shaare Zedek Medical Center, while the two boys were rushed to Hadassah Mount Scopus Medical Center.
Doctors at both hospitals were unable to save their lives.
MDA said that it was alerted that four people were hurt in the accident, though it did not give details about the fourth person, who may have been treated in the east of the city.
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The Israel Police said it opened an investigation into the incident.
Shuafat camp is the only Palestinian refugee camp that lies within the municipal boundaries of Jerusalem.
The road infrastructure is poor, with no sidewalks in many places.