A three-year-old boy died of swine flu Monday at the Schneider Medical Center in Petah Tikva.
Medical reports concluded that the child was not vaccinated against the flu.
This is the first death in Israel due to swine flu since 2009.
Four women were hospitalized this week after they were infected with the H1N1 virus. Two of them are currently in critical condition.
Health Ministry officials said last week that there is currently no special preparedness for the treatment of swine flu in Israel, but that the flu vaccine administered this year includes immunization against the life-threatening virus.
Children, the elderly, patients with chronic illnesses and pregnant women are considered to be at a high risk of contracting the disease.
Hundreds of cases of the H1N1 virus were recorded in the West Bank in recent weeks, with at least 10 people dying as a result of the illness, according to Israel Radio.